ABSTRACT
In vertebrates, the inflammatory reaction is responsible for modulating the initial nonspecific defense until specific immunity is acquired. In this context, numerous studies in mammals have demonstrated the participation of insulin in the inflammatory response, favoring cell proliferation and the migratory capacity of endothelial cells, vascular smooth muscle cells and monocytes, as well as mediating the expression of pro-thrombotic and pro-fibrotic factors. However, little is known about the effect of this peptidic hormone on the inflammatory reaction in teleostean fish. In order to evaluate the participation of insulin in the acute inflammatory response of Nile tilapia, Oreochromis niloticus, during aerocystitis induced by Aeromonas hydrophila, and 48 aloxane-diabetic tilapia were used, constituting two groups: diabetics treated with insulin and diabetics without treatment. After six, 24, and 48 hours of inflammatory stimulation, tilapia were submitted to deep anesthesia for euthanasia and necropsy, and thus, obtaining exudate and harvesting of the swim bladder for analysis of the inflammatory reaction. Based on this premise, the present study demonstrated the participation of insulin in the acute inflammatory reaction of alloxan-diabetic tilapia by favors the cellular accumulation in the exudate, the proliferative effect of fibrous tissue and neovascularization in the inflamed site. Such findings reinforce the old hypothesis that insulin plays an important role in the innate immune response during acute inflammatory reaction, being an important pro-inflammatory hormone. However, Nile tilapia proved to be a promising experimental model for studies and advances in research involving diabetes mellitus.(AU)
Em vertebrados, a reação inflamatória é responsável por modular a defesa inicial não-específica, até que imunidade específica seja adquirida. Neste contexto, inúmeros estudos em mamíferos têm demonstrado a participação da insulina sobre a resposta inflamatória, favorecendo a proliferação celular e a capacidade migratória das células endoteliais, células do músculo liso vascular e dos monócitos, além de mediar a expressão de fatores pró-trombótico e pró-fibrótico. Porém, pouco se conhece o efeito deste hormônio peptídico sobre a reação inflamatória em peixes teleósteos. Para avaliar a participação da insulina sobre a resposta inflamatória aguda em tilápias do Nilo, Oreochromis niloticus, na aerocistite induzida por Aeromonas hydrophila, foram utilizadas 48 tilápias aloxano-diabéticas, constituindo dois grupos: dos diabéticos tratados com insulina e diabéticos sem tratamento. Após, seis, 24 e 48 horas do estimulo inflamatório, as tilápias foram submetidas à anestesia profunda para eutanásia e necropsia, e assim, obtenção de exsudato e colheita da bexiga natatória para analise da reação inflamatória. Partindo-se desta premissa, o presente estudo demonstrou a participação da insulina na reação inflamatória aguda infecciosa de tilápias do Nilo aloxano-diabéticas por favorecer o acúmulo positivo celular no exsudato, assim como o efeito proliferativo de tecido fibroso e a neovascularização no local inflamado. Tais achados reforçam a hipótese de que a insulina desempenha importante papel na resposta imune inata na reação inflamatória aguda, sendo um importante hormônio pró-inflamatório. Contudo, a tilápia do Nilo demonstrou ser um modelo experimental promissor para estudos e avanços em pesquisas envolvendo o diabetes mellitus.(AU)
Subject(s)
Animals , Gram-Negative Bacterial Infections/veterinary , Aeromonas hydrophila/pathogenicity , Cichlids , Diabetes Mellitus, Experimental , InsulinABSTRACT
Objective To observe the efficacy and acute inflammatory reactionof Qingjin Huazhuo Prescription combined with Western medicine therapy for treatment of acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Methods Totally 60 hospitalized patients with AECOPD (Grade Ⅱ) and acute exacerbation course≤1 week were enrolled in this study. The patients were randomly divided into two groups, with 30 cases in each group. The control group was treated with conventional Western medicine. The observation group was treated with conventional Western medicine and oral administration of Qingjin Huazhuo Prescription for 10 days, once a day. TCM syndrome and dyspnea grading (mMRC score), white blood cell (WBC), C-reactive protein (CRP), procalcitonin (PCT) and partial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2), were measured before and after the treatment. Results The total scores of TCM syndromes and cough, expectoration, wheezing, fever, and cyanosis in the two groups decreased significantly (P<0.05). The improving degrees of cough and expectoration were better in the observation group than in the control group (P<0.05). The scores of mMRC in the two groups were better than before treatment (P<0.05). The levels of WBC, CRP and PaCO2 in the two groups were lower than those before treatment (P<0.05). The decreased degrees of CRP and PaCO2 in the observation group were better than those in the control group (P<0.05, P<0.01). Conclusion Qingjin Huazhuo Prescription combined with Western medicine therapy can relieve clinical symptoms, reduce the acute inflammatory reaction of AECOPD patients and improve respiration.
ABSTRACT
PURPOSE: Laparoscopy-assisted gastrectomy (LAG) is gaining wider acceptance as a minimally invasive treatment for early gastric cancer, but the safety, efficacy and clinical benefits of this type of surgery are still unclear. The purpose of this study is to compare laparoscopy-assisted gastrectomy (LADG) and conventional open distal gastrectomy (CODG) for early gastric cancer (EGC) according to the changes of the postoperative nutritional status and acute inflammatory reaction. MATERIALS AND METHODS: Eighty seven patients with EGC and who underwent a LADG between March 2006 and May 2009 at Daegu Catholic University Hospital, was enrolled. Over the same period, we enrolled 30 patients who underwent CODG and they were confirmed to have EGC from their pathology. The clinico-pathological features and serologic parameters were evaluated from the medical records and then retrospectively analyzed. RESULTS: There were no differences in the preoperative white blood cell (WBC), C-reactive protein (CRP) level, albumin level, the T4/T8 ratio and the other clinical data between the two groups. The total WBC counts gradually increased and they were significant lower at the 1st and 3rd postoperative days in the LADG group than that in the CODG group (P=0.001 and 0.008, respectively). The postoperative CRP levels were significantly lower at postoperative 5th day in the LADG group (P<0.001). The postoperative albumin and T4/T8 ratio gradually decreased, and the T4/T8 ratio was significantly higher at the 3rd postoperative day in the LADG group compared to that in the CODG group (P=0.003). CONCLUSION: This study demonstrates that the LADG has less of an influence on an acute inflammatory reaction than does CODG. Therefore, it is one of the safe and feasible procedures for the treatment of early gastric cancer.
Subject(s)
Humans , C-Reactive Protein , Gastrectomy , Leukocytes , Medical Records , Nutritional Status , Retrospective Studies , Stomach NeoplasmsABSTRACT
Objective To observe the neuroprotective effect of atorvastatin and its influence to tumor necrosis factor-alpha(TNF-α) and intedeukin-10(IL-10)in intraeerebral hemorrhage(ICH)rats,and to explore the possible mechanism of its neuroproteetive effect.Methods ICH WaS induced by stereotaxic infusion of collagenase into the right baSal ganglia,the expressions of TNF-α,IL-10 in the serum and brain tissue were analyzed by enzyme linked immunosorbent assay(ELISA),HE staining was used to evaluate the number of activated microglia.Results (1)The numbers of activated mieroglia,TNF-α and IL-10 were have significant differences between group A and group B(all P＜0.05).(2)In comparision with group B,numbers of activated mieroglia in group C,D and E were significantly redueed(P＜0.05).Atorvastatin cause a dose-dependent regulation,which decreased the level of TNF-α and inereased the level of IL-10 significantly(all P＜0.05).Conclusion The down-regulation of TNF-α and up-regulation of IL-10 might partly attribute to the neuropmtection of atovastatin.
ABSTRACT
Está establecido que la mejor técnica de reparación de las hernias incisionales consiste en el uso de mallas de polipropileno que permiten y proporcionan un mejor nivel de resistencia en la pared abdominal. El objetivo del presente trabajo es comparar las reacciones tisulares que resultan de la utilización de dos técnicas de implantación distintas, la implantación de la malla de polipropileno en el plano preaponeurótico (superficial) y la implantación de la malla de propileno en el plano preperitoneal (profundo). El estudio se realizó en 24 conejos de castilla empleando la primera técnica a la mitad de los conejos y la segunda a la otra mitad. Los resultados obtenidos a los 7, 14 y 21 días después de la cirugía fueron comparados microscópica y macroscópicamente, y concluimos que la técnica preaponeurótica presenta una menor Reacción Inflamatoria Aguda, mejor integración a la pared abdominal y mejor evolución clínica, en relación a la técnica preperitoneal.
It's has been known that the best repairing technique for incisional hernias consist in the use of polypropylene tights, which provides to abdominal wall a better resistance. The purpose if this Project is to compare tissue reactions that result of the use of these two implantation techniques, the implementation of the propylene tight on the preaponeurothic layer (superficial) and the implementation of the polypropylene tight on the preperitoneal layer (Deep). This study has been done in 24 rabbits using the first technique in half of the rabbits and the second technique in the other half The results that we have obtained at 7, 14 and 21 days after surgery, were compared microscope and macroscopically, and we conclude that preaponeurothic technique presents a less acule inflammatory reaction, better integration to the abdominal wall and better el clinical evolution, comparing to the preperitoneal technique.